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Literature summary extracted from

  • Gallego-Marin, C.; Schrum, J.E.; Andrade, W.A.; Shaffer, S.A.; Giraldo, L.F.; Lasso, A.M.; Kurt-Jones, E.A.; Fitzgerald, K.A.; Golenbock, D.T.
    Cyclic GMP-AMP synthase is the cytosolic sensor of Plasmodium falciparum genomic DNA and activates type I IFN in malaria (2018), J. Immunol., 200, 768-774 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.86 Plasmodium falciparum genomic DNA activates cGAS in vivo in THP-1 cells, no effect in enzyme-deficient cGAS-/- THP-1 cells. 2',3'-cGAMP is produced in Pf gDNA-transfected THP-1 cells but not in control THP-1 cells Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.86 cytosol
-
 Homo sapiens 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.86 Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.86 ATP + GTP Homo sapiens overall reaction 2 diphosphate + cyclic Gp(2'-5')Ap(3'-5')
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.86 Homo sapiens Q8N884
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.86 THP-1 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.86 ATP + GTP overall reaction Homo sapiens 2 diphosphate + cyclic Gp(2'-5')Ap(3'-5')
-
 ?
2.7.7.86 additional information quantification of cyclic Gp(2'-5')Ap(3'-5')(2',3'-cGAMP)WS is performed by LC-MS/MS Homo sapiens ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.7.7.86 cGAS
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.7.7.86 physiological function Plasmodium falciparum (Pf, strain 3D7) genomic DNA (gDNA) delivered to the cytosol of human monocytes (THP-1 cells) binds and activates the cyclic GMP-AMP synthase (cGAS). Activated cGAS synthesizes 2',3'-cGAMP, which acts as a second messenger for STING activation and triggers STING/TBK1/IRF3 activation, resulting in type I interferon (IFN) production in human cells. This induction of type I IFN is independent of IFI16. cGAS is an important cytosolic sensor of Plasmodium falciparum genomic DNA (Pf gDNA) and reveal the role of the cGAS/STING pathway in the induction of type I IFN in response to malaria parasites Homo sapiens